禽腺病毒多重TaqMan荧光定量PCR检测数据集
收稿日期: 2025-05-20
修回日期: 2025-08-07
网络出版日期: 2025-12-26
基金资助
中央级公益性科研院所基本科研业务费院级统筹项目：动物疫病数据中心;天津市科技计划项目(23YDTPJC00050);畜禽种质资源创新及高效繁育技术研究(2025ZYCX004)
Detection Dataset of Multiple Taq-Man Real-time PCR for Fowl Aviadenoviruses
Received date: 2025-05-20
Revised date: 2025-08-07
Online published: 2025-12-26
禽腺病毒自2015年在我国多省爆发后，给养禽业造成巨大损失。鸡群感染禽腺病毒后引起鸡心包积液-肝炎综合症、包涵体肝炎和肌胃糜烂等多种临床病症，且该病传播方式多样，发病没有明显的季节性，死淘率高（30%－80%），这些危害都给各养鸡场的生物安全防控水平提出了更多要求。如何保护鸡群体健康，保障养鸡场正常生产，是养鸡企业及相关科研单位关注的重点方向。本数据集包括临床样品采集数据、禽腺病毒12个血清型毒株的基因序列比较数据、荧光探针引物序列数据、禽腺病毒多血清型多重TaqMan荧光定量PCR检测方法数据及与行业标准同时检测161份临床样本数据集。数据显示此方法能特异性检测FAdV-4、FAdV-8a和FAdV-8b，与禽腺病毒其他血清型及NDV、AIVH9亚型、IBV等无交叉反应；最低检测值17 copies/μL；有良好的稳定性；与行业标准总阳性符合率为100.00%；天津及周边地区养鸡场禽腺病毒感染率偏低。本数据集可用于检测FAdV-4、FAdV-8a和FAdV-8b，为养鸡场禽腺病毒病的流行病学调查和早期快速筛查提供数据支撑，还可广泛用于养鸡场对其他疫病的生物安全防控技术的理论研究和实际应用。
数据摘要：
| 项目 | 描述 |
|---|---|
| 数据库（集）名称 | 禽腺病毒多重TaqMan荧光定量PCR检测数据集 |
| 所属学科 | 兽医学，家畜疾病学 |
| 研究主题 | 禽腺病毒多重TaqMan荧光定量PCR检测 |
| 数据时间范围 | 2023年－2024年 |
| 时间分辨率 | 1年 |
| 数据地理空间覆盖 | 天津及周边地区养鸡场 |
| 数据类型与技术格式 | .fas，.pdf，.xlsx |
| 数据库（集）组成 | 本数据集由5个数据组成，主要包括序列分析数据集、引物合成数据集、标准曲线扩增数据集、统计标准差平均值变异系数数据集、临床样品采集数据集。 |
| 数据量 | 1.51 MB |
| 主要数据指标 | 保守基因分析、荧光探针引物信息、标准曲线扩增数据信息、组内及组间重复性扩增数据信息、临床样品数据信息 |
| 数据可用性 | CSTR:17058.11.sciencedb.agriculture.00248；https://cstr.cn/17058.11.sciencedb.agriculture.00248 DOI:10.57760/sciencedb.agriculture.00248； |
| 经费支持 | 中央级公益性科研院所基本科研业务费院级统筹项目：动物疫病数据中心；天津市科技计划项目（23YDTPJC00050）；畜禽种质资源创新及高效繁育技术研究（2025ZYCX004）。 |
王利丽 , 师延峰 , 赵海明 , 李富强 , 崔松奇 , 郭笑然 , 李娥 , 孙慧中 , 李鹏飞 , 任卫科 , 田春利 , 鄢明华 . 禽腺病毒多重TaqMan荧光定量PCR检测数据集[J]. 农业大数据学报, 2025 , 7(4) : 532 -542 . DOI: 10.19788/j.issn.2096-6369.100063
Several serotypes of fowl aviadenoviruses were the aetiologic agent of hydro-pericardium-hepatitis syndrome (HHS), inclusion body hepatitis (IBH) and gizzard erosion (GE) in chicken farms. Outbreaks of these had been documented in multiple provinces of China in 2015, causing significant economic losses. Moreover, this disease had diverse transmission modes with no obvious seasonality, which was a mortality rate reported to be 30%-80%. All these had imposed more requirements on the biosecurity prevention and control level of various chicken farms. How to protect the health of the chicken flock and ensure the normal production of chicken farms was a key focus for chicken farms and related scientific research institutions. This dataset included clinical sample collection data, gene sequence comparison data of 12 serotypes of fowl aviadenoviruses, fluorescent probe primer sequence data, reaction system optimization data for the Multiple Taq-Man real-time PCR detection of fowl aviadenoviruses, and a data of 161 clinical samples tested simultaneously with industry standards. Data showed that this method could specifically detect FAdV-4, FAdV-8a and FAdV-8b, with no cross-reactivity with other serotypes of fowl aviadenoviruses, as well as NDV, AIV H9 subtype, IBV, etc. It had a minimum detection limit of 17 copies/μL and good stability, and the comparison result between the established method and the trade standard was 100.00%. In addition, the infection rate of fowl aviadenoviruses in chicken farms in Tianjin and surrounding areas was relatively low. This dataset could be used for the detection of FAdV-4, FAdV-8a and FAdV-8b, providing data support for epidemiological investigation and early rapid screening of this disease in chicken farms. It could also be widely applied in the theoretical research and practical application of biosafety prevention and control technologies for other epidemic diseases in chicken farms..
Data summary:
| Items | Description |
|---|---|
| Dataset name | Detection dataset of multiple Taq-Man real-time PCR for Fowl Aviadenoviruses |
| Specific subject area | Veterinary Medicine, Veterinary Pathology of Domestic Animals |
| Research topic | Detection of multiple Taq-Man real-time PCR for Fowl Aviadenoviruses |
| Time range | 2023-2024 |
| Temporal resolution | One year |
| Geographical scope | Chicken farms in Tianjin and its surrounding areas |
| Data types and technical formats | .fas，.pdf，.xlsx |
| Dataset structure | This dataset is composed of five data items, mainly including the sequence analysis dataset, the primer synthesis dataset, the standard curve amplification dataset, the dataset of statistical standard deviation, average value and coefficient of variation, and the clinical sample collection dataset. |
| Volume of dataset | 1.51 MB |
| Key index in dataset | Conserved gene analysis, fluorescence probe primer information, standard curve amplification data information, intra-group and inter-group repeatability amplification data information, clinical sample data information. |
| Data accessibility | CSTR:17058.11.sciencedb.agriculture.00248; https://cstr.cn/17058.11.sciencedb.agriculture.00248 DOI:10.57760/sciencedb.agriculture.00248; |
| Financial support | The Overall coordinated project of the Institute of The Fundamental Research Funds for the Central Public Welfare Research Institutes: Animal Epidemic Disease Data Center; the Science and Technology Planning Project of Tianjin (23YDTPJC00050); Innovative Research and Efficient Breeding Technology of Livestock and Poultry Germplasm Project (2025ZYCX004). |
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