乙草胺处理草莓线粒体膜参数检测数据集

doi:10.19788/j.issn.2096-6369.220316

农业大数据学报 ›› 2022, Vol. 4 ›› Issue (3): 96-102.doi: 10.19788/j.issn.2096-6369.220316

• 专题——果树育种数据 • 上一篇下一篇

乙草胺处理草莓线粒体膜参数检测数据集

鲁晓峰¹(), 孙海龙¹(), 杜国栋², 徐树广¹

^1.中国农业科学院果树研究所/农业农村部园艺作物种质资源利用重点实验室，兴城 125100
^2.沈阳农业大学园艺学院/沈阳市北方果树栽培与生理生态重点实验室，沈阳 110866

收稿日期:2022-07-29 出版日期:2022-09-26 发布日期:2022-12-29
通讯作者: 孙海龙 E-mail:13314021850@163.com;sunhailong@caas.cn
作者简介:[1] 鲁晓峰|鲁晓峰,女，硕士，助理研究员，研究方向:李杏资源与育种，E-mail:13314021850@163.com|孙海龙,杜国栋，等 . 乙草胺处理草莓线粒体膜参数检测数据集[DB/ OL]. 国家农业科学数据中心 .DOI:10.12205/A0015.20220721.10.ds.2210.|孙海龙,杜国栋，等 . 乙草胺处理草莓线粒体膜参数检测数据集[DB/ OL]. 国家农业科学数据中心 .DOI:10.12205/A0015.20220721.10.ds.2210.
基金资助:
中央级公益性科研院所基本科研业务费专项(1610182021004);中国农业科学院科技创新工程(CAAS-ASTIP-2021-ZFRI)

Dataset of Acetochlor Phytotoxicity on Mitochondrial Membrane Function in Strawberry Roots

Xiaofeng Lu¹(), Hailong Sun¹(), Guodong Du², Shuguang Xu¹

^1.Research Institute of Pomology, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization), Ministry of Agriculture and Rural Affairs, Xingcheng Liaoning 125100, China
^2.Shenyang Agricultural University, College of Horticulture, Shenyang, Liaoning Province, 110866, China

Received:2022-07-29 Online:2022-09-26 Published:2022-12-29
Contact: Hailong Sun E-mail:13314021850@163.com;sunhailong@caas.cn

摘要/Abstract

摘要：

乙草胺以选择性专一、持效期长及控制杂草效率高等诸多优点，在生产中被广泛应用，但在草莓繁育过程中的不当使用，对幼苗植株的生长发育产生一定的伤害。线粒体是感受外界信号的原初位点，为细胞内一切生理活动提供能量，同时也是产生ROS最多的部位，其膜结构和功能的变化与植物的耐受性紧密关联。然而目前对作物根系线粒体膜结构和功能的损伤机制研究较少，针对这一痛点，文章以草莓植株为试验材料，设置乙草胺处理及清水对照处理，测定处理后9 d内根系线粒体膜功能及保护性酶等相关指标的变化，探究乙草胺诱发ROS对草莓根系线粒体膜电位、膜通透性等生物膜结构和功能的影响，为生产上有效应对乙草胺类除草剂胁迫提供数据支撑。

关键词: 乙草胺, 草莓, 线粒体功能, 保护性酶, 除草剂

Abstract:

Owing to the specific selectivity, long persistence and effective weed control, acetochlor is widely used in agricultural production. However, the improper use of acetochlor in the strawberry breeding process can damage the growth and development of seedlings. Mitochondria is the primary site for sensing external signals, providing energy for all physiological activities in cells, and also the site producing the most ROS. Changes in membrane structure and function are closely related to plant tolerance. There are few studies on the damage mechanism of mitochondrial membrane structure and function in crop roots. In view of this pain point, Strawberry plants were used as experimental materials, the experiment comprised two treatments: an acetochlor treatment and a control treatment. Measured the changes of relevant indexes of root system within 9 days after treatment and study the effects of ROS induced by acetochlor on the structure and function of the mitochondrial membrane potential and membrane permeability in the roots of strawberry.

Key words: acetochlor, strawberry, function of the mitochondrial membrane, protective enzyme, herbicide

中图分类号:

S668

鲁晓峰, 孙海龙, 杜国栋, 徐树广. 乙草胺处理草莓线粒体膜参数检测数据集[J]. 农业大数据学报, 2022, 4(3): 96-102.

Xiaofeng Lu, Hailong Sun, Guodong Du, Shuguang Xu. Dataset of Acetochlor Phytotoxicity on Mitochondrial Membrane Function in Strawberry Roots[J]. Journal of Agricultural Big Data, 2022, 4(3): 96-102.

图/表 5

图1

图2

图3

图4

参考文献 35

1	Mclnenly L E, Merrill E H, Cahill J F, et al. Festuca campestris alters root morphology and growth in response to simulated grazing and nitrogen form[J]. Functional Ecology, 2010, 24(2): 283 - 292.
2	宋宏峰, 郭磊, 张斌斌,等. 除草剂对毛桃幼苗生长与光合的影响[J]. 园艺学报, 2014, 41(11):2208-2214.
	Song H F, Guo L, Zhang B B,et al. Effects of Herbicides on Growth and Photosynthesis of Prunus persica Seedlings[J]. Acta Horticulturae Sinica, 2014, 41(11): 2208-2214.
3	李亚东, 梁友, 韩小强,等. 四种土壤处理除草剂对棉花叶片氧化应激防御系统及根系活力的影响[J]. 石河子大学学报(自然科学版), 2016, 34(06): 703-708.
	Li Y D, Ling Y, Han X Q,et al. Effects of Four Kinds of Soil- applied Herbicides on Oxidative Stress Defense System and Root Activity of Cotton[J]. Journal of Shihezi University: Natural Science, 2016, 34(06): 703-708.
4	程华, 李琳玲, 常杰,等. 植物抗氧化酶的研究进展[C]. 中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集, 2008: 766-773.
	Cheng H, Li L L, Chang J,et al. Advances in Research on Antioxidant Enzymes in Plants[C]. Modern gardening Forum, 2008: 766-773.
5	张玉秀, 柴团耀. 植物耐重金属机理研究进展[J]. 植物学报, 1999, 41(5): 453-457.
	Zhang Y X, Chai T Y. Research Advances on the Mechanisms of Heavy Metal Tolerance in Plants[J]. Acta Botanica Sinica, 1999, 41(5): 453-457.
6	张蜀宁, 张振超, 张红亮,等. 低温胁迫对不同倍性不结球白菜生长及生理生化特征的影响[J]. 西北植物学报, 2008, 28(1): 109-112.
	Zhang S N, Zhang Z C, Zhang H L,et al. Growth, Physiological and Biochemical Characteristics of Non-heading Chinese Cabbage Under Low Temperature Stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(1): 109-112.
7	Jiang L, Yang Y, Jia L X, et al. Biological Responses of Wheat (Triticum aestivum) Plants to The Herbicide Simetryne in Soils [J]. Ecotoxicology & Environmental Safety, 2016, 127: 87-94.
8	Gogoi AK, Kalita H. Effect of Seeding Method and Herbicide on Weeds and Growth and Yield of Wheat[J]. Indian Journal of Agronomy, 1992, 40(2): 209-211.
9	Chen H, Xu Q M, Wang W, et al. The Effect of Cd ²⁺ on the Activity of Protect and Cell Membrane Lipid Peroxidation Change of Wheat Seedlings [J]. Acta Bot Boreal - OccidSin, 2000, 20(3): 399-403.
10	温银元,郭平毅,尹美强,等.扑草净对远志幼苗根系活力及氧化胁迫的影响[J].生态学报, 2012, 32(8): 2506-2514.
	Wen Y Y, Guo P Y, Yin M Q, et al. Effect of Prometryne on Root Activity and Oxidative Stress of Polygala Tenuifolia Willd. Seedling Roots[J]. Acta Ecologica Sinica, 2012, 32(8): 2506-2514.
11	冯煜. 除草剂复配安全剂对糜子根系活性氧代谢及土壤环境的影响研究[D]. 西北农林科技大学, 2021.
	Feng Y. Effects of Herbicide Compounded with Safeners on Active Oxygen Metabolism in Root System and Soil Environment of Proso Millet[D]. Northwest A＆F University, 2021.
12	Cadenas E, Davies K J A. Mitochondrial Free Radical Generation, Oxidative Stress, and Aging[J]. Free Radical Biology & Medicine, 2000, 29(3-4): 222-230.
13	Ma H, L D, Liu G, et al. Mitochondrial Response in theApical and Lateral Flower Buds of the Hanfu Apple to Cold Stress During the Dormancy Stage[J]. Acta Ecologica Sinica, 2013, 33(1): 52-58.
14	卫星, 王政权, 张国珍. 干旱胁迫下水曲柳苗木细根线粒体的形态及活性变化[J]. 植物生态学报, 2010, 34(12): 1454-1462.
	Wei X, Wang Z Q, Zhang G Z. Morphological and Activity Variation of Mitochondria in Fine Roots of Fraxinus Mandshurica Seedling Under Drought Stress[J]. Chinese Journal of Plant Ecology, 2010, 34(12): 1454-1462.
15	潘雄波, 向丽霞, 胡晓辉,等. 外源亚精胺对盐碱胁迫下番茄幼苗根系线粒体功能的影响[J]. 应用生态学报, 2016, 27(2): 491-498.
	Pan X B, Xiang L X, Hu X H, et al. Effects of Exogenous Spermidine on Mitochondrial Function of Tomato Seedling Roots Under Salinity-alkalinity Stress[J]. Chinese Journal of Applied Ecology, 2016, 27(2): 491-498.
16	徐萍, 李进, 吕海英,等. 干旱胁迫对银沙槐幼苗叶绿体和线粒体超微结构及膜脂过氧化的影响[J]. 干旱区研究, 2016, 33(1): 120-130.
	Xu P, Li J, Lv H Y, et al. Effects of Drought Stress on Ultrastructure of Chloroplast and Mitochondriaand Membrane Lipid Peroxidation of Ammodendron Argenteum. Arid Zone Reaserch, 2016, 33(1): 120-130.
17	姜倩倩, 林家燕, 杨槟瑜. 钙对镉胁迫下平邑甜茶根系活性氧生成和细胞损伤的影响[J]. 潍坊学院学报, 2019, 19(6): 23-28.
	Jiang Q Q, Lin J Y, Yang B Y. The Role of Calcium in the Generation of Reactive Oxygen Species and Cell Damage in Roots of Malus hupehensis Rehd. Under Cadmium Stress[J]. Journal of Weifang University, 2019, 19(6): 23-28.
18	陈丽, 陆晓晔, 朱长清. 百草枯致线粒体损伤关系的研究进展[J]. 中华急诊医学杂志, 2011(9): 1002-1004.
	Chen L, Lu X Y, Zhu C Q. Research on the Relationship Between Paraquat Induced Mitochondrial Damage[J]. Chinese Journal of Emergency Medicine, 2011(9): 1002-1004.
19	张召, 梁元存, 王利,等. 钙对酸化处理平邑甜茶根系抗氧化酶活性及线粒体功能的影响[J]. 林业科学, 2012, 48(8): 87-93.
	Zhang Z, Liang Y C, Wang L, et al. Effect of Calcium on the Functions of Antioxidant Systems and Mitochondria in Malus hupehensis var.pingyiensis Roots Under Acid Treatment[J]. Scientia Silvae Sinicae, 2012, 48(8): 87-93.
20	Castello P R, Drechsel D A, Manisha P. Mitochondria Are A Major Source of Paraquat-induced Reactive Oxygen Species Production in the Brain.[J]. Journal of Biological Chemistry, 2007, 282(19): 14186-14193.
21	Ichas F, Mazat J P. From Calcium Signaling to Cell Death: Two Conformations for the Mitochondrial Permeability Transition Pore, Switching from Low- to High-conductance State[J]. Biochimica Biophysica Acta, 1998, 1366(2): 33-50.
22	鲁晓峰, 杜国栋, 邵静,等. 草莓根系线粒体对外源酚酸胁迫的生理响应[J]. 中国农业科学, 2021, 54(5): 1029-1042.
	Lu X F, Du G D, Sao J, et al. Physiological Response of Mitochondrial Function of Strawberry Roots to Exogenous Phenolic Acid[J]. Scientia Agricultura Sinica, 2021, 54(5): 1029-1042.
23	徐建兴. 细胞色素C在线粒体中的抗氧化功能[J]. 中国科学院院刊, 2003, 18(4): 277-278.
	Xu J X. The Antioxidative Function of Cytochrome C in Mitochondria[J]. Chinese Academy of Sciences,2003,18(4): 277-278.
24	Yang J Y, Tan Y P, Xia C J, et al. Honglian Cytoplasmic Male Sterility in Relation to Its Mitochondrial Permeability Transition[J]. Journal of Wuhan Botanical Research, 2004, 22(5): 385-390.
25	Braidot E, Petrussa E, Macrì F, et al. Plant Mitochondrial Electrical Potential Monitored by Fluorescence Quenching of Rhodamine 123[J]. Biologia Plantarum,1998,41(2): 193-201.
26	金超芳, 沈生荣, 赵保路. EGCG对线粒体PT孔开放及Ca²⁺转运的影响[J]. 茶叶科学, 2002, 22(1): 14-18.
	Jin C F, Shen S R, Zhao B L. Influences of EGCG on Mitochondrial PTP Opening and Ca2+ Transportation. Journal of Tea Science, 2002, 22(1): 14-18.
27	Tonshin A A, Saprunova V B, Solodovnikova I M, et al. Functional Activity and Ultrastructure of Mitochondria Isolated from Myocardial Apoptotic Tissue[J]. Biochemistry, 2003, 68(8): 875-881.
28	Ohkawa H, Ohishi N, Yagi K, et al. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction[J]. Analytical Biochemistry, 1979, 95(2): 351-358.
29	李合生. 植物生理生化实验原理和技术[M]. 高等教育出版社, 2000.
	Li H S. Principles and Techniques of Plant Physiological and Biochemical Experiments. Higher Education Press, 2000.
30	Giannopolitis C N, Ries S K. Purification and Quantitative Relationship with Water-soluble Protein in Seedling[J]. Plant Physiol, 1977, 59: 315-318.
31	Dhindsa R S, Thorpe T A. Leaf senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase[J]. Journal of Experimental Botany, 1981, 32(1): 93-101.
32	汤章城. 现代植物生理学实验指南[M]. 科学出版社, 1999.
	Tang Z C. Experimental Guide to Modern Plant Physiology. Science Press, 1999.
33	Uchida A, Jagendorf A T, Hibino T, et al. Effects of Hydrogen Peroxide and Nitric Oxide on Both Salt and Heat Stress Tolerance in Rice[J]. Plant Science, 2002, 163(3): 515-523.
34	王学奎, 章文华, 郝再彬. 植物生理生化原理与技术［M］. 北京: 高等教育出版社,2006.
	Wang X K, Zhang W H, Hao Z B. Principles and techniques of plant physiology and biochemistry experiment. Beijing：Higher Education Press, 2006.
35	Blumwald E, Poole R J. Salt Tolerance in Suspension Cultures of Sugar Beet. Induction of Na⁺/H⁺ Antiport at the Tonoplast by Growth in Salt[J]. Plant Physiology, 1987，83: 884-887.

数据库（集）名称	2017年乙草胺处理草莓线粒体膜参数检测数据集
数据作者及分工	鲁晓峰,田间调查、数据处理、质量控制及论文撰写;孙海龙,方案设计、组织实施、论文构架及质量控制;杜国栋,方案设计、组织实施、论文构架及质量控制;徐树广,田间调查及质量控制
通讯作者及邮箱	孙海龙， E-mail: sunhailong@caas.cn
数据时间范围	2017年
数据量	36.3 kb
数据格式	*.xlsx
数据服务系统网址	http://www.agridata.cn/data.html
基金项目	中央级公益性科研院所基本科研业务费专项（1610182021004）；中国农业科学院科技创新工程（CAAS-ASTIP-2021-ZFRI）
数据库（集）组成	数据集由1个excel文件组成，为2017年乙草胺药害对草莓根系线粒体膜功能伤害分析数据集，包括草莓根系线粒体膜电位、细胞色素Cyt c/a、线粒体膜透性、根系活力及H⁺ - ATPase、丙二醛及活性氧含量数据。

乙草胺处理草莓线粒体膜参数检测数据集

Dataset of Acetochlor Phytotoxicity on Mitochondrial Membrane Function in Strawberry Roots

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 35

相关文章 1

Metrics

本文评价

推荐阅读 0