Research Progress on Breeding Techniques of Main Fruit Trees in Northern China

Expand
  • Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China

Received date: 2022-07-12

  Online published: 2022-12-29

Abstract

Breeding technology is an important tool for fruit tree breeding. Fruit tree breeding techniques mainly include conventional breeding techniques such as cross breeding, mutation and seedling selection, and auxiliary breeding techniques including radiation breeding, molecular marker assisted breeding, and genetic transformation. At present, conventional breeding technology is the main technology for breeding major fruit trees in northern China, and most varieties are selected through conventional breeding techniques. Mutation breeding and tissue culture are important auxiliary breeding techniques that play valuable roles in fruit breeding in northern China. Molecular marker-assisted breeding and genetic transformation technologies were used, molecular markers related to important traits were screened and developed, and important transgenic fruit trees were obtained, which laid an important foundation for molecular breeding of fruit trees. Using apple, pear, grape, and peach as examples, this paper summarized the breeding techniques of major fruit trees in northern China, introduced the characteristics and application of breeding techniques, and addressed the future of fruit tree breeding strategies and methods to provide ideas and references for the breeding major fruit trees in northern China.

Cite this article

Fei Wang, Chunqing Ou, Yanjie Zhang, Haibo Wang, Li Ma, Shuling Jiang . Research Progress on Breeding Techniques of Main Fruit Trees in Northern China[J]. Journal of Agricultural Big Data, 2022 , 4(3) : 6 -14 . DOI: 10.19788/j.issn.2096-6369.220301

References

1 陈学森,王楠,张宗营,等. 关于果树种质资源与遗传育种若干问题的理解与思考[J]. 中国农业科学, 2022, 55(17): 3395-3410.
1 Chen X S, Wang N, Zhang Z Y, et al. Understanding and Thinking About Some Problems of Fruit Tree Germplasm Resources and Genetic Breeding[J]. Scientia Agricultura Sinica, 2022, 55(17): 3395-3410.
2 邓秀新,王力荣,李绍华,等. 果树育种40年回顾与展望[J]. 果树学报, 2019, 36 (4): 514-520.
2 Deng X X, Wang L R, Li S H, et al. Retrospective and prospect of fruit breeding for the last four decades in China[J]. Journal of Fruit Science, 2019, 36 (4): 514-520.
3 满书铎,牛健哲,丛佩华,等. 苹果晚熟新品种‘华红’的选育[J]. 中国果树, 1999, (1): 13-14.
3 Man S D, Niu J Z, Cong P H, et al. Breeding of a New Late maturing Apple Variety 'Huahong' [J]. China Fruits, 1999, (1): 13-14.
4 阎振立,张恒涛,过国南,等. 苹果新品种——华硕的选育[J]. 果树学报, 2010, 27(4): 655-656.
4 Yan Z L, Zhang H T, Guo G N, et al. Selection of a new early-ripening apple cultivar—Huashuo[J]. Journal of Fruit Science, 2010, 27(4): 655-656.
5 杨亚州,赵政阳,高华,等. 晚熟苹果新品种‘瑞香红’[J]. 园艺学报,2021, 48(3): 609-610.
5 Yang Y Z, Zhao Z Y, Gao H, et al. A New Late Ripening Apple Cultivar‘Ruixianghong’ [J]. Acta Horticulturae Sinica, 2021, 48(3): 609-610.
6 邹养军,马锋旺,符轩畅,等. 晚熟苹果新品种‘秦脆’[J]. 园艺学报,2019,46(5):1011-1012.
6 Zou Y J, Ma F W, Fu X C, et al. A New Late Ripening Apple Cultivar‘Qincui’[J]. Acta Horticulturae Sinica, 2019,46(5):1011-1012.
7 姜淑苓. 中国早酥梨[M].北京:中国农业科学技术出版社, 2018.
7 Jiang S L. ZhongGuo ZaoSu Li[M].Beijing: China Agricultural Science and Technology Press, 2018.
8 王迎涛,李勇,孙荫槐. 中熟抗黑星病梨新品种黄冠的选育及应用[J]. 河北农业科学,1998,2(2): 40-42.
8 Wang Y T, Li Y, Sun Y H. The breeding and application of the middle ripening, scab resistant new pear variety—Huangguan pear[J]. Journal of Hebei Agricultural sciences, 1998,2(2): 40-42.
9 郭黄萍,李晓梅,张建功. 优质中熟红梨新品种‘玉露香’[J]. 山西果树,2001,(1): 3-4.
9 Guo H P, Li X M, Zhang J G. A New Red Pear Variety 'Yuluxiang' with High Quality and Medium Maturity[J]. Shanxi Fruits, 2001,(1): 3-4.
10 施泽彬,过鑫刚. 早熟砂梨新品种翠冠的选育及其应用[J]. 浙江农业学报, 1999, 11(4): 212-214.
10 Shi Z B, Guo X G. The breeding of early ripening pear variety Cuiguan and its application[J]. Acta Agriculturae Zhejiangensis, 1999, 11(4): 212-214.
11 徐卫东. 极早熟三倍体无核葡萄新品种夏黑及栽培技术[J]. 山西果树,2003,(3): 21-22.
11 Xu W D. Xiahei, a new seedless triploid grape variety with very early maturity, and its cultivation techniques [J].Shanxi fruits, 2003,(3): 21-22.
12 王玉环,姜学品,王桂华,等. 葡萄新品种巨玫瑰的选育[J]. 中国果树, 2003,(1): 3-6.
12 Wang Y H, Jiang X P, Wang G H, et al. Breeding of a new grape variety Jumeigui[J]. China Fruits, 2003,(1): 3-6.
13 王海波,王宝亮,冀晓昊,等. 早熟鲜食葡萄新品种‘华葡紫峰’[J]. 园艺学报,2019,46 (S2):2753-2754.
13 Wang H B, Wang B L, Ji X H, et al. A New Early-ripening Table Grape Cultivar‘Huapu Zifeng’[J]. Acta Horticulturae Sinica, 2019,46 (S2):2753-2754.
14 陈昌文,王力荣,朱更瑞,等. 中熟蟠桃新品种‘中蟠19号’的选育[J]. 果树学报,2020,37(4): 610-612.
14 Chen C W, Wang L R, Zhu G R, et al. A new middle ripening flat peach cultivar‘Zhongpan 19’ [J]. Journal of Fruit Science, 2020,37(4): 610-612.
15 王力荣,方伟超,陈昌文,等. 早中熟油蟠桃新品种‘中油蟠9号’的选育[J]. 果树学报,2020,37(6): 942-944.
15 Wang L R, Fang W C, Chen C W, et al. A new early-middle flat nectarine peach cultivar‘Zhongyoupan 9’ [J]. Journal of Fruit Science, 2020,37(6): 942-944.
16 鲁振华,牛良,崔国朝,等. 早熟黄肉桃新品种‘黄金蜜桃1 号’的选育[J]. 果树学报,2020,37(9): 1434-1436.
16 Lu Z H, Niu L, Cui G C, et al. An early ripening and yellow flesh peach variety‘Huangjinmitao 1’ [J]. Journal of Fruit Science, 2020,37(9): 1434-1436.
17 陈学森, 王楠, 张宗营, 等. 关于果树种质资源与遗传育种若干问题的理解与思考[J]. 中国农业科学, 2022,55(17):3395-3410.
17 Chen X S, Wang N, Zhang Z Y, et al. Understanding and Thinking About Some Problems of Fruit Tree Germplasm Resources and Genetic Breeding[J]. Scientia Agricultura Sinica, 2022,55(17):3395-3410.
18 王爱玲,田时敏,梁哲军,等. 我国苹果育种的研究现状及展望[J].中国果菜,2020,40(12): 60-62.
18 Wang A L, Tian S M, Liang Z J, et al. Research Status and Prospect of Apple Breeding in China[J]. China Fruit & Vegetable, 2020,40(12): 60-62.
19 王斐,姜淑苓,欧春青,等. 我国梨育种亲本的选配及骨干亲本分析[J].中国南方果树,2015,44(5):106-111.
19 Wang F, Jiang S L, Ou C Q, et al. Selection of parents for pear breeding in china and analysis of key parents[J]. South China Fruits, 2015,44(5):106-111.
20 焦言英,赵桂敏,陈继忠. 南果梨芽变新品种红南果梨[J].中国果树,1999,(3):22.
20 Jiao Y Y, Zhao G M, Chen J Z. A New Bud Variety of Nanguo Pear, Hongnanguo[J]. China Fruits, 1999,(3):22.
21 章镇,俞宏,高志红,等. 梨新品种‘大果黄花’[J]. 园艺学报,2000,27(1):74.
21 Zhang Z, Yu H, Gao Z H, et al. Pear ‘Daguohuanghua’[J]. Acta Horticulturae Sinica, 2000,27(1):74.
22 廖明安,张志鹏,任雅君,等. 优质高产梨新品种‘川花梨’[J]. 园艺学报,2010,37(6):1017-1018.
22 Liao M A, Zhang Z P, Ren Y J, et al. A New Good Quality and High Yield Pear Cultivar‘Chuanhua Li’ [J]. Acta Horticulturae Sinica, 2010,37(6):1017-1018.
23 王浩,刘崇怀,樊秀彩,等. 葡萄芽变机制研究进展及应用[J]. 果树学报,2022,39(3):474-482.
23 Wang H, Liu C H, Fan X C, et al. Advances in research on bud mutantation mechanism in grape[J]. Journal of Fruit Science,2022,39(3):474-482.
24 陈学森,郭文武,徐娟,等. 主要果树果实品质遗传改良与提升实践[J]. 中国农业科学, 2015, 48(17): 3524-3540.
24 Chen X S, Guo W W, Xu J, et al. Genetic Improvement and Promotion of Fruit Quality of Main Fruit Trees[J]. Scientia Agricultura Sinica, 2015, 48(17): 3524-3540.
25 赵兰英,雷世俊,韩宪东,等. 桃新品种‘处暑蜜’的选育[J].中国果树,2018,(6):70-71.
25 Zhao L Y, Lei S J, Han X D, et al. Breeding of a New Peach Variety 'Chushu Honey'[J].China Fruits, 2018,(6):70-71.
26 吴远峰. 优质芽变早熟大果油桃——金油608(暂定名)[J]. 山西果树,2018,(4):20-21.
26 Wu Y F. High Quality Budding and Early Ripening Nectarine Variety of Big Fruit—Jinyou 608(Temporary Name) [J]. Shanxi Fruits, 2018,(4):20-21.
27 闫君君,刘明,杨海清,等. 桃芽变新品种‘谷丰’的选育[J]. 北京农学院学报,2017,32(1):24-27.
27 Yan J J, Liu M, Yang H Q, et al. ‘Gufeng’ a new peach cultivar from bud sport of ‘Okubo’ peach[J]. Journal of Beijing University of Agriculture, 2017,32(1):24-27.
28 文露,王永清,邓群仙,等. 皮球桃黄肉芽变的ISSR和SRAP分子标记鉴定[J]. 基因组学与应用生物学, 2020, 39(11): 5180-5185.
28 Wen L, Wang Y Q, Deng Q X, et al. Identification of a yellow-flesh sport of Piqiutao peach (prunus persica) using ISSR and SRAP markers[J]. Jiyinzuxue Yu Yingyong Shengwuxue (Genomics and Applied Biology), 2020, 39(11): 5180-5185
29 沈德绪.果树育种学[M].上海:上海科学技术出版社,1986.
29 Shen D X. Fruit Breeding[M].Shanghai: Shanghai Science and Technology Press, 1986.
30 孔庆山. 中国葡萄志[M].北京:中国农业科学技术出版社, 2004.
30 Kong Q S. Grapes of China[M]. Beijing: China Agricultural Science and Technology Press,2004.
31 姜建福,孙海生,刘崇怀,等. 2000年以来中国葡萄育种研究进展[J]. 中外葡萄与葡萄酒,2010,(3):60-65+69.
31 Jiang J F, Sun H S, Liu C H, et al. Advances in grape breeding in china since 2000[J].Sino-overseas grapevine &wine,2010,(3):60-65+69.
32 丛佩华,张彩霞,韩晓蕾,等. 我国苹果育种研究现状及展望[J].中国果树,2018,(6):1-5.
32 Cong P H, Zhang C X, Han X L, et al. Current research situation and prospect of apple breeding in China[J].China Fruits, 2018,(6):1-5.
33 马之胜,贾云云,王越辉,等. 大久保桃在我国桃育种中的应用研究进展[J]. 河北农业科学,2006,10(4):103-105.
33 Ma Z S, Jia Y Y, Wang Y H, et al. Research Progress on Application of Okubo Peach in Peach Breeding in China[J]. Journal of Hebei Agricultral Sciences,2006,10(4):103-105.
34 肖啸,张立彬,李清泉,等. 桃新品种——‘久鲜’的选育[J]. 果树学报,2014,31(4):745-746.
34 Xiao X, Zhang L B, Li Q Q, et al.‘Jiuxian’, a new peach cultivar from seedling of ‘Okubo’ peach[J]. Journal of Fruit Science,2014,31(4):745-746.
35 刘进,杨成发,王慧,等. 桃晚熟黄肉新品种‘金黄金’的选育[J]. 中国果树,2016,(4):72-73.
35 Liu J, Yang C F, Wang H, et al. Breeding of a New Peach Variety 'Jin Huangjin' with Late Ripening and Yellow Flesh[J].China Fruits, 2016,(4):72-73.
36 王虞英.桃的实生选种和杂交育种[J].北京农业科学,1984,(6):29-31.
36 Wang Y Y. Seedling Selection and Cross Breeding of Peach[J]. Beijing Agricultural Sciences, 1984,(6):29-31.
37 闻玉. 抗寒苹果新品系——‘宁光’[J]. 宁夏农业科技,1986,(5):16.
37 Wen Y. A New Cold resistant Apple Line — 'Ningguang'[J]. Ningxia Journal of Agriculture Science and Technology, 1986,(5):16.
38 袁志成.苹果新品中——‘宁富’培育研究报告[J]. 宁夏农林科技,1992,(1):19-21.
38 Yuan Z C. Research Report on the Breeding of Apple variety 'Ningfu' [J]. Ningxia Journal of Agriculture and Forestry Science and Technology,1992,(1):19-21.
39 冯永利. γ射线诱变苹果新品种——东垣红[J]. 青海农林科技,1995,(2):64.
39 Feng Y L. A New Apple Variety—Dongyuanhong Induced by γ-rays[J]. Science and Technology of Qinghai Agriculture and Forestry,1995,(2):64.
40 张敏. 无锈金冠——岳金[J]. 落叶果树,1991,(3):57.
40 Zhang M. Rust Free Golden Delicious—Yue Jin[J]. Deciduous Fruits,1991,(3):57.
41 孟玉平,曹秋芬,杨承建,等. 梨新品种——晋巴梨[J]. 园艺学报, 2008,35(3):461.
41 Meng Y P, Cao Q F, Yang C J, et al. A New Pear Cultivar—Jinbali[J]. Acta Horticuluturar Sinica, 2008,35(3):461.
42 胡钟东,闫承璞,曾明,等. 砂梨辐射育种试验初报[J]. 现代园艺,2013,(2):6-7.
42 Hu Z D, Yan C P, Zeng M, et al. Preliminary report on radiation breeding of sand pear[J]. Contemporary Horticulture,2013,(2):6-7.
43 罗耀武. 四倍体‘玫瑰香’及‘巨玫’葡萄的选育及遗传研究[J]. 云南大学学报(自然科学版), 1999,(S3):112-113.
43 Luo Y W. Breeding and Inheritance of Tetraploid Grapes' Rose Fragrance 'and' Ju Mei '[J]. Journal of Yunnan University(Natural Sciences Edition), 1999,(S3):112-113.
44 马小河,唐晓萍,陈俊,等. 优质中熟葡萄新品种‘秋黑宝’[J].园艺学报,2010,37(11):1875-1876.
44 Ma X H, Tang X P, Chen J, et al. A New Excellent Mid-maturing Grape Cultivar‘Qiuheibao’[J]. Acta Horticulturae Sinica,2010,37(11):1875-1876.
45 陈俊,唐晓萍,李登科,等. 早熟大粒优质葡萄新品种——早黑宝[J]. 园艺学报,2001,28(3):277.
45 Chen J, Tang X P, Li D K, et al.‘Zaoheibao’ —An Early-maturing Good Quality and Large Berry Grape Variety[J]. Horticulturae Sinica, 2001,28(3):277.
46 李树举,涂超峰,周振林,等. 观赏桃辐射诱变育种研究初报[J]. 核农学报,2010,24(2):287-291.
46 Li S J, Tu C F, Zhou Z L, et al. Preliminary Study on Irradiation Breeding of Ornamental Peach[J]. Journal of Nuclear Agricultural Sciences,2010,24(2):287-291.
47 梁绍信. CO2激光诱变早熟水蜜桃育成‘沙激1号’和‘沙激2号’两品种[J]. 云南农业大学学报,1986,1(1):71-80.
47 Liang S X. ‘Shaji No.1’ and ‘Shaji No.2’ The new breeding cultivars of early honeydew peach induced by CO2 Laser[J]. Journal of Yunnan Agricultural University,1986,1(1):71-80.
48 牛健哲,薛光荣,丛佩华,等. 应用花药培养技术培育苹果新类型[J]. 果树科学,1994,11(1):1-4.
48 Niu J Z, Xue G R, Cong P H, et al. Anther culture induced apples[J]. Journal of Fruit Science,1994,11(1):1-4.
49 杨振英,薛光荣,苏佳明,等. 苹果新品种华富选育研究[J]. 中国果树,2005,(1):1-3.
49 Yang Z Y, Xue G R, Su J M, et al. Study on the Breeding of a New Apple Variety Huafu[J]. China Fruits,2005,(1):1-3.
50 薛光荣,杨振英,史永忠,等. 锦丰梨花粉植株的诱导[J]. 园艺学报,1996,23(2):123-127.
50 Xue G R, Yang Z Y, Shi Y Z, et al. Induction of pollen plantlets by anther culture in vitro in ‘Jin Feng’ Pear[J]. Acta Horticuluturar Sinica,1996,23(2):123-127.
51 邹昌杰,李佩芬. 葡萄(Vitis vinifera L.)花粉植株的诱导[J]. 植物学报,1981,23(1):79-81.
51 Zou C J, Li P F. Induction of pollen plants of grape (Vitis vinifera L.)[J]. Acta Botanica Sinica,1981,23(1):79-81.
52 顾爱侠,冯大领,轩淑欣,等. 胚挽救技术在植物育种中的应用[J]. 河北林果研究,2010,25(1):30-33.
52 Gu A X, Feng D L, Xuan S X, et al. Application of embryo rescue technology in plant breeding[J]. Hebei Journal of Forestry and Orchard Research,2010,25(1):30-33.
53 潘春云,亓桂梅,汤小宁,等.葡萄三倍体育种初报[J].山东农业大学学报,1998,29(3):299-302.
53 Pan C Y, Qi G M, Tang X N, et al. Primary Report on Grape-Triploid Breeding[J]. Journal of Shandong Agricultural University,1998,29(3):299-302.
54 徐海英,阎爱玲,张国军. 葡萄二倍体与四倍体品种间杂交胚珠的离体培养[J].果树学报,2001,18(6):317-320.
54 Xu H Y, Yan A L, Zhang G J. Study on the in ovulo embryo culture of the crossed progeny between diploid and tetraploid grape cultivars[J]. Journal of fruit science,2001,18(6):317-320.
55 郭印山,郭修武,张海娥,等. 利用胚挽救技术获得三倍体葡萄植株研究[J]. 沈阳农业大学学报,2005,36(5): 606- 608.
55 Guo Y S, Guo X W, Zhang H E, et al. Studies on Acquir ing Tr iploid Gr ape with Embryo Rescue Techniques[J]. Journal of Shenyang Agricultural University,2005,36(5): 606- 608.
56 马瑞娟,俞明亮,杜平,等. 油桃早熟新品种紫金红1号的选育[J]. 中国果树,2009,(6):3-5.
56 Ma R J, Yu M L, Du P, et al. Breeding of A New Early Maturing Nectarine Variety Zijinhong 1[J]. China Frutis, ,2009,(6):3-5.
57 陈昌文,朱更瑞,王力荣,等. 蟠桃新品种‘中蟠桃10号’的选育[J]. 果树学报,2015,32(2):339-340.
57 Chen C W, Zhu G R, Wang L R, et al. A new flat peach variety‘Zhong Pantao 10’ [J]. Journal of Fruit Science,2015,32(2):339-340.
58 牛良,鲁振华,崔国朝,等.早熟、优质油蟠桃新品种中油蟠36-3的选育[J]. 果树学报,2022,39(5):895-898.
58 Niu L, Lu Z H, Cui G C, et al. An early ripening and hiyh quality flat nectarine cultivar Zhong Youpan 36-3[J]. Journal of Fruit Science, 2022,39(5):895-898.
59 张朝红,杨凤秋,陈东玫,等. 短枝型苹果的遗传分析及分子标记筛选[J]. 河北农业大学学报,2018,41(5):80-83.
59 Zhang C H, Yang F Q, Chen D M, et al. Genetic studies and molecular markers screening of spur-type apple[J]. Journal of Hebei Agricultural university,2018,41(5):80-83.
60 王雷存,樊红科,高华,等. 苹果酸度基因( MaSSR 标记及遗传分析[J]. 园艺学报,2012,39(10):1885-1892.
60 Wang L C, Fan H K, Gao H, et al. Genetic Analysis and SSR Markers of Acid Gene Ma in Apple[J]. Acta Horticulturae Sinica,2012,39(10):1885-1892.
61 何晓薇,王彩虹,田义轲,等. 苹果果皮颜色性状的SSR标记[J]. 果树学报,2009,26(3):379-381.
61 He X W, Wang C H, Tian Y K, et al. A SSR marker related to the traits of skin color in apple[J]. Journal of Fruit Science,2009,26(3):379-381.
62 宋伟,王彩虹,田义轲,等. 梨果实形状的SSR分子标记[J]. 青岛农业大学学报(自然科学版), 2010,27(3):213-215.
62 Song W, Wang C H, Tian Y K, et al. Development of SSR Molecular Markers Linked to the Fruit Shape in Pear[J]. Journal of Qingdao Agricultural University (Natural Science), 2010,27(3):213-215.
63 田义轲,王彩虹,贾彦利,等. 梨矮化基因pcDw的SSR标记定位[J]. 果树学报,2008,25(3):404-407.
63 Tian Y K, Wang C H, Jia Y L, et al. Location of a pear dwar f gene pcDw by SSR marker[J]. Journal of Fruit Science,2008,25(3):404-407.
64 王龙,王苏珂,薛华柏,等.梨栽培相关性状的QTL分析[J]. 果树学报,2018,35():61-65.
64 Wang L, Wang S K, Xue H B, et al. QTL analysis for cultivated traits in pears[J]. Journal of Fruit Science,2018,35:61-65.
65 王力荣,郭健,曹珂,等.与桃果形圆、扁性状连锁的SNP位点、基于该位点的分子标记及其应用[P].中国:CN104962627B. 2019.
65 Wang L R, Guo J, Cao K, et al.SNP locus in linkage with round or flat character of peach fruit shape,molecular marker based on locus and application[P]. China:CN104962627B, 2019.
66 孟君仁,曾文芳,邓丽,等. 桃若干重要性状的KASP分子标记开发与应用[J]. 中国农业科学, 2021,54(15):3295-3307.
66 Meng J R, Zeng W F, Deng L, et al. Development and Application of KASP Molecular Markers of Some Important Traits for Peach[J]. Scientia Agricultura Sinica, 2021,54(15):3295-3307.
67 王跃进, Olusola Lamikanra. 检测葡萄无核基因DNA探针的合成与应用[J]. 西北农林科技大学学报(自然科学版),2002,30(3):42-46.
67 Wang Y J, Olusola Lamikanra. Application and synthesis on the DNA probe for detecting seedless genes in grapevine[J]. Journal of Northwest A & F University(Natural Science Edition),2002,30(3):42-46.
68 王跃进,张剑侠,周鹏,等. 中国野生葡萄抗白粉病基因的RAPD标记[J]. 西北农林科技大学学报(自然科学版),2001,29(1):1-5.
68 Wang Y J, Zhang J X, Zhou P, et al. RAPD marker of resistance gene to Uncinula necator in Chinese wild Vitis [J]. Journal of Northwest A & F University(Natural Science Edition), 2001,29(1):1-5.
69 徐炎,王跃进,周鹏,等. 中国野生葡萄果实抗白腐病基因RAPD标记的克隆及序列分析[J]. 西北农林科技大学学报(自然科学版),2002,30(6):85-88.
69 Xu Y, Wang Y J, Zhou P, et al. Cloning and sequencing of RAPD maker linked to white rot disease resistant gene in wild grapes native to China[J]. Journal of Northwest A & F University(Natural Science Edition),2002,30(6):85-88.
70 张艳艳,张剑侠,王跃进. 中国野生葡萄抗霜霉病基因RAPD标记的筛选[J]. 果树学报,2008,25(6):816-820.
70 Zhang Y Y, Zhang J X, Wang Y J. Screening the RAPD markers linked to the gene resistant to downy mildew in Chinese wild species of Vitis [J]. Journal of Fruit Science,2008,25(6):816-820.
71 张剑侠,王跃进,张艳艳,等.中国野生葡萄抗黑痘病基因RAPD标记的克隆、序列分析及辅助育种应用[J]. 果树学报,2009,26(4):456-460.
71 Zhang J X, Wang Y J, Zhang Y Y,et al. Cloning and sequence analysis of the RAPD marker linked to anthracnose-resistance gene in Chinese wild Vitis and its application for markerassisted breeding[J]. Journal of Fruit Science, 2009,26(4):456-460.
72 李心悦,张克闯,张道远,等. 新疆野苹果瞬时遗传转化方法建立及初步验证[J]. 分子植物育种,2018,16 (22):7315-7321.
72 Li X Y, Zhang K C, Zhang D Y, et al. Establishment and Preliminary Validation of Transient Genetic Transformation Method of Malus sieversii [J]. Molecular Plant Breeding,2018,16 (22):7315-7321.
73 陶爱群. 豆梨离体再生体系建立与rolB基因转化研究[D]. 长沙:湖南农业大学, 2008.
73 Tao A Q. Establishment of in vitro regeneration system of Pea pear and transformation of rolB gene [D]. Changsha:Hunan Agricultural University, 2008.
74 王海燕. 农杆菌介导rolB 基因转化杜梨的研究[D]. 保定: 河北农业大学, 2014.
74 Wang H Y. Agrobacterium-mediated transformation of rolB gene into Du pear [D]. Baoding: Hebei Agricultural University,2014.
75 李桂琴,齐靖,高志华,等. 鸭梨多酚氧化酶基因反义表达载体的构建及农杆菌介导的遗传转化[J]. 植物遗传资源学报,2010,11(5):635-639.
75 Li G Q, Qi J, Gao Z H, et al. Construction of polyphenol oxidase gene antisense expression vector and Agrobacterium- mediated genetic transformation[J]. Journal of Plant Genetic Resources,2010,11(5):635-639.
76 吴延军,张上隆,谢鸣,等. 桃ACO基因反义转化桃幼胚子叶的研究[J]. 遗传, 2006,28(1):65-70.
76 Wu Y J, Zhang S L, Xie M, et al. Genetic transformation of peach immature cotyledons with its antisense ACO gene[J].Yi Chuan,2006,28(1):65-70.
77 张亚林,李唯,张伟,等. 根癌农杆菌介导反义PG基因对桃的遗传转化[J]. 甘肃农业大学学报,2010,45(6):55-59.
77 Zhang Y L, Li W, Zhang W, et al. Genetic transformation of peach with antisense PG gene by Agrobacterium tumef aciens [J]. Journal of Gansu Agricultural University,2010,45(6):55-59.
78 刘昱. 葡萄体细胞胚的诱导及VvACS1基因对葡萄的遗传转化[D]. 郑州:河南农业大学, 2018.
78 Liu Y. Induction of somatic embryos and transformation of VvACS1 gene in grape[D]. Zhengzhou: Henan Agricultural University, 2018.
79 鲍睿. 中国野生毛葡萄VqRSGT 基因的遗传转化与功能研究[D]. 杨凌:西北农林科技大学, 2017.
79 Bao R. A study on the genetic transformation and functional analysis of VqRSGT from Chinese wild Vitis Quinquangularis [D]. Yangling: Northwest Agricultural and Forestry University, 2017.
80 Zhang L, Hu J, Han X, et al. A high-quality apple genome assembly reveals the association of a retrotransposon and red fruit colour[J]. Nat. Commun., 2019, 10(1): 1-13.
81 Dong X G, Wang Z, Tian L M, et al. De novo assembly of a wild pear (Pyrus betuleafolia) genome[J]. Plant Biotechnol. J., 2020, 18(2): 581-595.
82 Chunqing Ou, Fei W, Jiahong W, et al. A de novo genome assembly of the dwarfing pear rootstock Zhongai 1[J]. Scientific Data, 2019, 6:281 | https://doi.org/10.1038/s41597-019-0291-3.
83 Roach M J, Johnson D L, Bohlmann J, et al. Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay[J]. PLoS Genet, 2018, 14(11): e1007807.
84 Aranzana M J, Decroocq V, Dirlewanger E, et al. Prunus genetics and applications after de novo genome sequencing: achievements and prospects[J]. Hortic. Res., 2019, 6(1): 1-25.
85 倪海枝,王引,颜帮国,等. 果树基因组辅助育种技术研究现状与展望[J]. 分子植物育种, .
85 Ni H Z, Wang Y, Yan B G, et al. Research Status and Prospects of Genomics-Assisted Breeding Technology in Fruit Trees[J]. Molecular Plant Breeding. .
Outlines

/