Unreasonable fertilization problems such as excessive fertilization and partial fertilization are common in grape production, which seriously restricts the healthy and sustainable development of the grape industry. Soil testing and formula fertilization technology is an important way to achieve rational and precise fertilization. Proving the soil background nutrients in grape-producing areas is a necessary condition to guide formula fertilization. In this study, soil samples were collected in the vineyards of Beizhen, Gaizhou, Liaoyang, Huludao Taijitun in the four main grape production areas of Kyoho grapes in Liaoning Province for three consecutive years from 2019 to 2021 during the annual fruit ripening period. Six indicators of soil nitrate nitrogen content, ammonium nitrogen content, available phosphorus content, available potassium content, exchangeable calcium content and exchangeable magnesium content in each sample were determined. Mainly used electronic balance, automatic flow chemistry analyzer, flame spectrophotometer and automatic atomic absorption spectrometer for measurement. The dataset includes the soil available macroelements content of more than 100 vineyard areas in the four main producing areas of Kyoho grapes in Liaoning Province, which can provide data support for the formula fertilization of local Kyoho grapes, thereby promoting efficient nutrient management of Kyoho grapes and realizing green and high-quality development of Kyoho grapes in Liaoning Province.