Organic fertilizer production is mainly based on livestock and poultry manure raw materials, which is one of the important sources of antibiotic-contaminated soil. It is of great significance to explore the impact of antibiotic residues on the ecological environment of organic fertilizer, especially the impact of the "organic fertilizer-soil-crop" pathway, which is of great significance for the safe application of organic fertilizer. This data set collected a total of 116 samples of sheep manure, cow manure, livestock and poultry manure raw materials and commercial organic fertilizers using livestock and poultry manure as the main raw materials in different regions of Inner Mongolia, including 74 organic fertilizer raw materials and 42 finished products, and the content of 4 tetracyclic antibiotics was detected to evaluate the possibility of tetracyclic antibiotic risks in different livestock and poultry manure and different regions, so as to provide a basis for the safety risk monitoring and evaluation of regional organic fertilizers. The data showed that the detection rate of raw material oxytetracycline was 8.62%, tetracycline 10.34%, chloromycin 10.34%, doxycycline 1.72%, commodity organic fertilizer chloromycin 2.58%, doxycycline 0.86%, and the detection rate of raw antibiotics was higher than that of commercial organic fertilizer.