邻苯二甲酸二丁酯和邻苯二甲酸单丁酯对菠菜生长的毒性效应及累积特征数据集

doi:10.19788/j.issn.2096-6369.100021

Abstract

Abstract:

The phthalate esters (PAEs) has been one of the prevalent organic pollutants in agricultural soils due to the extensive use of mulch in agricultural production processes in China. Monobutyl phthalate (MBP), monoethylhexyl phthalate (MEHP) and other phthalate monoesters (MPEs) are important intermediates in the metabolic process of PAEs and co-exist with PAEs in the soil environment. The toxicological studies have shown that these metabolites have equal or even stronger endocrine disrupting effects. Therefore, the co-occurrence of MPEs in soil could eventually increase the human health risk through the food chain. In addition, the accumulation of MPEs caused by the continuous metabolism of PAEs in soil also has potential toxic effects on crops. In this paper, spinach was selected as the test plant, and the effects of DBP and MBP on the growth, photosynthetic indexes, quality and antioxidant system of spinach were investigated by setting up six different concentrations of dibutyl phthalate (DBP) and MBP to observe seed germination within two weeks and three different concentrations of MBP to focus on the pot planting experiments to obtain the relevant datasets, and at the same time, the absorption and transport datasets of DBP and MBP in spinach were analyzed in a comparative manner. At the same time, the uptake and transport data of DBP and MBP in spinach were analyzed comparatively, aiming to investigate the toxic effects of MBP on the growth of spinach and provide the corresponding data sets for the cumulative characteristics, so as to provide a reference basis for the comprehensive assessment of the ecological risk of PAEs and their metabolite contamination in soil.

Data summary:

Item	Description
Dataset name	Toxic Effects and Cumulative Characteristics of Dibutyl Phthalate and Monobutyl Phthalate on Spinach Datasets
Specific subject area	Agricultural science,Resource utilization and plant protection
Research topic	Toxic effects and cumulative characteristics of dibutyl phthalate and monobutyl phthalate on spinach datasets
Time range	2020-2023
Geographical scope	Planting area of Qingdao Agricultural University, Chengyang District, Qingdao, Shandong Province, China
Data types and technical formats	Spinach Charts，.JPG；Spinach flux coefficient，.TXT；SPSS21.0*.JPG；
Dataset structure	The dataset is composed of 25 image files and 6 text type files. The image files are JPG files. The text files are TXT files.
Volume of dataset	353.07 kB
Key index in dataset	Seed germination, photosynthetic indicators, flux coefficient, cumulative uptake
Data accessibility	DOI： https://doi.org/10.57760/sciencedb.agriculture.00106 CSTR： https://cstr.cn/17058.11.sciencedb.agriculture.00106

Key words: dibutyl phthalate, monobutyl phthalate, spinach, seed germination, physiology, photosynthesis, uptake and accumulation, datasets

QI ShuShu, LIU XiaoChen. Toxic Effects and Cumulative Characteristics of Dibutyl Phthalate and Monobutyl Phthalate on Spinach Datasets[J].Journal of Agricultural Big Data, 2025, 7(1): 132-140.

Figures/Tables 12

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References 14

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[9]	张小红, 王亚娟, 陶红, 等. 宁夏土壤中PAEs污染特征及健康风险评价. 中国环境科学, 2020, 40(9):3930-3941.
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仪器名称	仪器型号	生产厂家
高效液相色谱仪	LC20A	日本岛津公司
气相色谱串联四级杆质谱联用仪	TSQ 8000 Evo	美国赛默飞世尔科技有限公司
旋转蒸发仪	RE-52A	上海亚荣生化仪器厂
生化培养箱	LRH-250	上海一恒科学仪器有限公司
循环水式真空泵	SHB -III	郑州长城科工贸有限公司
便携式植物光合作用仪	CIRAS-3	美国PP Systems公司
超声波清洗仪	KQ-600B	昆山市超声仪器有限公司
箱式电阻炉	SX2-4-10D	龙口市电炉制造厂
电热鼓风干燥箱	GZX-9070MBE	上海博讯事业有限公司医疗设备厂

测量指标	测定方法^[13-14]
SOD活性 CAT活性	氮蓝四唑光化还原法紫外吸收法含量
POD活性	愈创木酚法
MDA含量	硫代巴比妥酸法
叶绿素a、叶绿素b、类胡萝卜素含量	利用分光光度计测定叶绿素提取液在最大波长下的吸光度，即朗伯-比尔定律计算提取液中各个叶绿素的含量
可溶性糖含量	蒽酮法
可溶性蛋白含量	考马斯蓝染色法
维生素C含量	2,6-二氯靛酚法

指标	测定方法	表达方式
脲酶	苯酚钠-次氯酸钠比色法	24 h后1 g土壤中NH3-N的质量
磷酸酶	磷酸苯二钠比色法	24 h后1 g土壤中释放出的酚的质量
蔗糖酶	3,5-二硝基水杨酸比色法	24 h后1 g干土生成葡萄糖质量
过氧化氢酶	高锰酸钾滴定法	20 min内1 g土壤分解的过氧化氢的毫克数

	芽长			根长
	回归方程	R²	EC₅₀	回归方程	R²	EC₅₀
DBP	I=0.361C_DBP+8.583	0.994	114.72	I=0.457 C_DBP +9.275	0.989	89.11
MBP	I=0.302 C_MBP +5.299	0.983	148.02	I=0.380 C_MBP +3.811	0.984	121.55

处理浓度(mg/kg)		光合速率[mg/(dm²·h)]	气孔导度[mmol/(m²·s)]	蒸腾速率[g/(m²·h)]	细胞间CO₂浓度(μmol/L)
CK	0	12.57a	80.67a	1.83a	249.67c
DBP	5	12.23a	66.33b	1.43abc	260.33bc
	20	11.43ab	52.33cd	1.27c	282.67ab
	100	9.23c	48.33d	1.17c	301.33a
MBP	5	12.13a	76.33a	1.73ab	259.67bc
	20	12.17a	53.33c	1.47abc	266.33ab
	100	9.97bc	49.33cd	1.33bc	295.33a

Toxic Effects and Cumulative Characteristics of Dibutyl Phthalate and Monobutyl Phthalate on Spinach Datasets

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处理浓度(mg/kg)		叶绿素 (mg/g)	叶绿素a (mg/g)	叶绿素b (mg/g)	类胡萝卜素(mg/g)
CK		2.25a	1.58a	0.44a	0.34a
DBP	5	2.10b	1.546a	0.44a	0.32b
	20	1.89c	1.39bc	0.36c	0.27c
	100	1.63d	1.186d	0.32d	0.26c
MBP	5	2.17ab	1.56a	0.42ab	0.30b
	20	2.14ab	1.43b	0.40b	0.27c
	100	1.80c	1.32c	0.35c	0.26c

	脲酶	磷酸酶	蔗糖酶	过氧化氢酶
DBP	-0.849**	-0.815**	-0.559	-0.940**
MBP	-0.787**	-0.748**	-0.404	-0.858**

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