禽腺病毒多重TaqMan荧光定量PCR检测数据集

doi:10.19788/j.issn.2096-6369.100063

Abstract

Abstract:

Several serotypes of fowl aviadenoviruses were the aetiologic agent of hydro-pericardium-hepatitis syndrome (HHS), inclusion body hepatitis (IBH) and gizzard erosion (GE) in chicken farms. Outbreaks of these had been documented in multiple provinces of China in 2015, causing significant economic losses. Moreover, this disease had diverse transmission modes with no obvious seasonality, which was a mortality rate reported to be 30%-80%. All these had imposed more requirements on the biosecurity prevention and control level of various chicken farms. How to protect the health of the chicken flock and ensure the normal production of chicken farms was a key focus for chicken farms and related scientific research institutions. This dataset included clinical sample collection data, gene sequence comparison data of 12 serotypes of fowl aviadenoviruses, fluorescent probe primer sequence data, reaction system optimization data for the Multiple Taq-Man real-time PCR detection of fowl aviadenoviruses, and a data of 161 clinical samples tested simultaneously with industry standards. Data showed that this method could specifically detect FAdV-4, FAdV-8a and FAdV-8b, with no cross-reactivity with other serotypes of fowl aviadenoviruses, as well as NDV, AIV H9 subtype, IBV, etc. It had a minimum detection limit of 17 copies/μL and good stability, and the comparison result between the established method and the trade standard was 100.00%. In addition, the infection rate of fowl aviadenoviruses in chicken farms in Tianjin and surrounding areas was relatively low. This dataset could be used for the detection of FAdV-4, FAdV-8a and FAdV-8b, providing data support for epidemiological investigation and early rapid screening of this disease in chicken farms. It could also be widely applied in the theoretical research and practical application of biosafety prevention and control technologies for other epidemic diseases in chicken farms..

Data summary:

Items	Description
Dataset name	Detection dataset of multiple Taq-Man real-time PCR for Fowl Aviadenoviruses
Specific subject area	Veterinary Medicine, Veterinary Pathology of Domestic Animals
Research topic	Detection of multiple Taq-Man real-time PCR for Fowl Aviadenoviruses
Time range	2023-2024
Temporal resolution	One year
Geographical scope	Chicken farms in Tianjin and its surrounding areas
Data types and technical formats	.fas，.pdf，.xlsx
Dataset structure	This dataset is composed of five data items, mainly including the sequence analysis dataset, the primer synthesis dataset, the standard curve amplification dataset, the dataset of statistical standard deviation, average value and coefficient of variation, and the clinical sample collection dataset.
Volume of dataset	1.51 MB
Key index in dataset	Conserved gene analysis, fluorescence probe primer information, standard curve amplification data information, intra-group and inter-group repeatability amplification data information, clinical sample data information.
Data accessibility	CSTR:17058.11.sciencedb.agriculture.00248; https://cstr.cn/17058.11.sciencedb.agriculture.00248 DOI:10.57760/sciencedb.agriculture.00248; https://doi.org/10.57760/sciencedb.agriculture.00248
Financial support	The Overall coordinated project of the Institute of The Fundamental Research Funds for the Central Public Welfare Research Institutes: Animal Epidemic Disease Data Center; the Science and Technology Planning Project of Tianjin (23YDTPJC00050); Innovative Research and Efficient Breeding Technology of Livestock and Poultry Germplasm Project (2025ZYCX004).

Key words: fowl aviadenoviruses, the Multiple Taq-Man real-time PCR, rapid screening, Detection dataset

WANG LiLi, SHE YanFeng, ZHAO HaiMing, LI FuQiang, CUI SongQi, GUO XiaoRan, LI E, SUN HuiZhong, LI PengFei, REN WeiKe, TIAN ChunLi, YAN MingHua. Detection Dataset of Multiple Taq-Man Real-time PCR for Fowl Aviadenoviruses[J].Journal of Agricultural Big Data, 2025, 7(4): 532-542.

Figures/Tables 9

Table 1

Table 2

Table 3

Fig. 1

Table 4

Fig. 2

Fig. 3

Table 5

Table 6

References 24

[1]	朱增勇, 李梦希, 张学彪. 非洲猪瘟对中国生猪市场和产业发展影响分析. 农业工程学报, 2019, 35(18):205-210.
	ZHU Z Y, LI M X, ZHANG X B. Analysis on effects of African swine fever on China's pig market and industry development. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2019, 35(18): 205-210.
[2]	ANGEL E A, ANDRÉS M G, PEDRO F V-H, et al. Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses. Virology, 2017, 15(501):63-69.
[3]	NIU D, FENG J, DUAN B, et al. Epidemiological survey of avian adenovirus in China from 2015 to 2021 and the genetic variability of highly pathogenic Fadv-4 isolates. Infection, Genetics and Evolution, 2022, 101:105277-105290. doi: 10.1016/j.meegid.2022.105277
[4]	王建琳, 江之瑶, 栾庆东, 等. 我国主要流行的4种血清型禽腺病毒-Ⅰ群感染SPF鸡的致病性比较研究. 畜牧兽医学报, 2022, 53(5): 1598-1607. doi: 10.11843/j.issn.0366-6964.2022.05.027
	WANG J L JIANG Z Y LUAN Q D, et al. Comparative pathological analysis of SPF chicken host infected with serotype 4,8a, 8b and 11 fowl adenovirus popular in China. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5):1598-1607.
[5]	NIU Y, SUN Q, ZHANG G, et al. Epidemiological investigation of outbreaks of fowl adenovirus infections in commercial chickens in China. Transboundary and Emerging Diseases, 2018, 65(1): e121-126. doi: 10.1111/tbed.2018.65.issue-1
[6]	ZHANG H, JIN W, DING K, et al. Genetic characterization of fowl adenovirus strains isolated from poultry in China. Avian Diseases, 2017, 61(3):341-346. doi: 10.1637/11621-030817-RegR pmid: 28956993
[7]	ZHANG T, JIN Q, DING P, et al. Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China. Virology Journal, 2016, 13(1): 188-195. pmid: 27863494
[8]	ZHUANG Q Y, WANG S CH, ZHANG F Y, et al. Molecular epidemiology analysis of fowl adenovirus detected from apparently healthy birds in eastern China. BMC Veterinary Research, 2023, 19(1): 5-13. doi: 10.1186/s12917-022-03545-5 pmid: 36624468
[9]	LV L, LU H, WANG K, et al. Emerging of a novel natural recombinant fowl adenovirus in China. Transboundary and Emerging Diseases, 2021, 68(2):283-288. doi: 10.1111/tbed.v68.2
[10]	吴双, 张聪, 袁慧莎, 等. 血清4型禽腺病毒TaqMan探针实时荧光定量PCR检测方法的建立与应用. 江苏农业学报, 2023, 39(1): 134-141.
	WU SH, ZHANG C, YUAN H S, et al. Establishment and application of Quantitative Real-time PCR detection method for fowl aviadenovirus serotype 4 based on TaqMan probe. Jiangsu Journal of Agricultural Sciences, 2023, 39(1):134-141.
[11]	UGWU C C, HAIR-BEJO M, NURULFIZA M I, et al. TaqMan probe-based qPCR method for specific detection and quantification of fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Veterinary Journal, 2023, 13(2): 171-178. doi: 10.5455/OVJ.2023.v13.i2.4 pmid: 37073244
[12]	WANG J CH, WANG J F, CHEN P, et al. Development of a TaqMan-based real-time PCR assay for rapid and specific detection of fowl aviadenovirus serotype 4. Avian Pathology, 2017, 46(3): 338-343. doi: 10.1080/03079457.2016.1278428 pmid: 28437156
[13]	中华人民共和国农业行业标准. . 北京: 中国农业出版社, 2022.
	Agricultural Industry Standards of the People's Republic of China. [S]. Beijing: China Agricultural Press, 2022.
[14]	LI P H, ZHENG P P, ZHANG T F, et al. Fowl adenovirus serotype 4: Epidemiology, pathogenesis, diagnostic detection, and vaccine strategies. Poultry Science, 2017, 96(8): 2630-2640. doi: 10.3382/ps/pex087 pmid: 28498980
[15]	冯敬敬, 牛登云, 马利芳, 等. 2015-2020年我国禽腺病毒流行病学调查及高致病性FAdV-4分离株遗传变异性分析. 中国动物传染病学报, 2021, 29(5): 92-98.
	FENG J J, NIU D Y, MA L F, et al. Epidemiological survey of avian adenovirus in China from 2015 to 2020 and analysis of genetic variability of highly pathogenic FAdV-4 isolates. Chinese Journal of Animal Infectious Diseases, 2021, 29(5): 92-98.
[16]	CHEN S L, LIN F Q, JIANG B, et al. Isolation and characterization of a novel strain of duck aviadenovirus B from Muscovy ducklings with acute hepatitis in China. Transboundary and Emerging Diseases, 2022, 69(5):2769-2778. doi: 10.1111/tbed.v69.5
[17]	WEI Z P, LIU H, DIAO Y, et al. Pathogenicity of fowl adenovirus (FAdV) serotype 4 strain SDJN in Taizhou geese. Avian Pathology, 2019, 48(5):477-485. doi: 10.1080/03079457.2019.1625305 pmid: 31155930
[18]	XUE X Y, YANG Q H, WU M J, et al. Genomic and pathologic characterization of the first FAdV-C serotype 4 isolate from Black-Necked Crane. Viruses, 2023, 15(8):1653-1663. doi: 10.3390/v15081653
[19]	卢清侠, 金前跃, 冯丽丽, 等. 禽腺病毒血清4型 SYBR Green Ⅰ荧光定量PCR 检测方法的建立与应用. 河南农业科学. 2022, 51(12): 131-138.
	LU Q X, JIN Q Y, FENG L L, et al. Development and application of SYBR Green Ⅰ-Based Real-time PCR assay method for detection of fowl adenovirus serotype 4. Journal of Henan Agricultural Sciences, 2022, 51(12):131-138.
[20]	刘琳, 何春辉, 王赛楠, 等. 血清4型禽腺病毒TaqMan实时荧光定量PCR检测方法的建立. 中国兽医学报, 2020, 40(5):928-932.
	LIU L, HE C H, WANG S N, et al. Development of a TaqMan Real-time PCR assay for detection of serum 4 fowl adenovirus. Chinese Journal of Veterinary Science, 2020, 40(5):928-932.
[21]	窦砚国. 血清4型Ⅰ群禽腺病毒的分离鉴定及流行病学调查. 泰安: 山东农业大学, 2017.
	DOU Y G. Isolation, Identification and Epidemoological Investigation of Group Ⅰ Foul Adenovirus Serotype 4. Tai'an, Shandong Agricultural University, 2017.
[22]	YUAN X Y, WANG Y L, MENG K, et al. LAMP real-time turbidity detection for fowl adenovirus. BMC Veterinary Research, 2019, 15(1): 256-259. DOI: 10.1186/s12917-019-2015-5.
[23]	周珈羽, 朱春华, 陈珍, 等. 禽腺病毒DAdV-3和FAdV-4双重荧光定量PCR检测方法的建立及应用. 中国兽医学报, 2024, 44(1): 66-73.
	ZHOU J Y, ZHU C H, CHEN Z, et al. Establishment and application of a duplex fluorescence quantitative PCR for detection of DAdvV-3 and FAdV-4. Chinese Journal of Veterinary Science, 2024, 44(1): 66-73.
[24]	殷冬冬, 郭浩, 兰梦蝶, 等. 重组酶辅助扩增结合CRISPR/Cas13a检测禽腺病毒4型方法的建立. 云南农业大学学报(自然科学), 2023, 38(5): 803-807.
	YIN D D, GUO H, LAN M D, et al. Detection of fowl adenovirus serotype 4 by recombinase aided amplification combined with CRISPR/Cas13a. Journal of Yunnan Agricultural University (Natural Science), 2023, 38(5):803-807.

采样区域	采样地点	样品类型及数量
采样区域	采样地点	疑似发病鸡组织样品	健康鸡泄殖腔拭子	鸡场环境拭子
天津武清区	A	5	6	5
	B	8	5	5
	C	7	6	6
天津宝坻区	D	6	5	4
	E	5	5	6
	F	4	4	4
天津静海区	G	6	6	8
天津静海区	H	5	4	6
天津蓟州区	I	5	5	6
天津周边养殖场	J	5	4	5

毒株	种属	血清型	登陆号	毒株	种属	血清型	登陆号
CELO	FAdV-A	FAdV-1	U46933.1	"A-2A	FAdV-D	FAdV-9	NC_000899.1
FAdV-A	FAdV-A	FAdV-1	NC_001720.1	ON NP2	FAdV-D	FAdV-11	KP231537.1
FAdV-A	FAdV-A	FAdV-1	AC000014.1	/	FAdV-D	/	AC_000013.1
JM1/1	FAdV-A	FAdV-A	MF168407.1	SR48	FAdV-D	FAdV-2	KT862806.1
OTE	FAdV-A	FAdV-A	MK572847.1	685	FAdV-D	FAdV-2	KT862805.1
340	FAdV-B	FAdV-5	NC_021221.1	SR49	FAdV-D	FAdV-3	KT862807.1
LYG	FAdV-B	FAdV-5	MK757473.1	A-2A	FAdV-D	FAdV-9	AF083975.2
B1-7	FAdV-C	FAdV-4	KU342001.1	380	FAdV-D	FAdV-11	KT862812.1
AHFY19	FAdV-C	FAdV-10	MN542422.1	CR119	FAdV-E	FAdV-6	NC_038332.1
MX-SHP95	FAdV-C	FAdV-4	KP295475.1	YR36	FAdV-E	FAdV-7	KT862809.1
ON1	FAdV-C	FAdV-4	GU188428.1	TR59	FAdV-E	FAdV-8a	KT862810.1
/	FAdV-C	FAdV-4	NC_015323.1	764	FAdV-E	FAdV-8b	KT862811.1
GX-1	FAdV-C	FAdV-4	MH454598.1	HeB20	FAdV-E	FAdV-8b	OK188966.1
KR5	FAdV-C	FAdV-4	HE608152.1

引物及探针	序列（5'→3')	产物大小（bp）	目的基因
FAdV4-F	AACTCTACACCAACAACACCGC	192	hexon
FAdV4-R	GTCGAAGCCGGAGATGC
FAdV4-P	FAM-TTCTACTTCGGCACGGTACCCTCCTA-BHQ1
FAdV8a-F	CTACAACTCCCAACGGTCTTAC	119	Fiber
FAdV8a-R	CCGGACGTAAGATAGGCATTG
FAdV8a-P	ROX-TCACCGTAAATGCCGGGACCTTAA-BHQ2
FAdV8b-F	AACCATCACGGGACAAATGAC	164	hexon
FAdV8b-R	CTGTGCTGCTAGTGTCGGGGT
FAdV8b-P	HEX-CTGCAGCAATCGCCAGCGTTTCAG-BHQ1

目的病原	荧光信号	斜率	扩增效率 (%)	R²	Y轴截距
FAdV-4	FAM	-3.311	100.461	1.000	37.703
FAdV-8a	ROX	-3.386	97.382	1.000	38.378
FAdV-8b	HEX	-3.315	100.274	1.000	38.194

目的病原	质粒浓度(copies/µL)	组内重复性试验结果		组间重复性试验结果
目的病原	质粒浓度(copies/µL)	平均值±标准差 Mean±SD	变异系数 CV%	平均值±标准误 Mean±SD	变异系数 CV%
FAdV-4	1.7×10²	30.876±0.311	1.0	30.640±0.295	1.0
	1.7×10⁴	24.269±0.192	0.8	24.110±0.255	1.1
	1.7×10⁶	17.448±0.096	0.6	17.394±0.148	0.9
FAdV-8a	1.7×10²	31.538±0.024	0.1	31.430±0.212	0.7
	1.7×10⁴	24.681±0.059	0.2	24.580±0.120	0.5
	1.7×10⁶	17.413±0.154	0.9	17.586±0.174	1.0
FAdV-8b	1.7×10²	31.600±0.066	0.2	31.410±0.187	0.6
	1.7×10⁴	24.816±0.136	0.5	24.620±0.187	0.8
	1.7×10⁶	17.303±0.135	0.8	17.325±0.181	1.0

Detection Dataset of Multiple Taq-Man Real-time PCR for Fowl Aviadenoviruses

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 24

Related Articles 0

Metrics

Comments

Recommended 0

样品总数（份）	病原	多重荧光定量PCR方法检测结果		行业标准检测结果		总阳性符合率 (%)
样品总数（份）	病原	阳性数	阳性率 (%)	阳性数	阳性率 (%)	总阳性符合率 (%)
161	FAdV-4	16	9.94	18	11.18	100.00
	FAdV-8a	1	0.62
	FAdV-8b	1	0.62