云南地方小麦种质资源条锈病抗性鉴定与抗病基因分子检测

doi:10.19788/j.issn.2096-6369.230205

Abstract

Abstract:

In-depth identification of crop germplasm resources is the premise and basis for breeding and utilization. The Crop Genebank of Yunnan Province has preserved abundant wheat germplasm resources in Yunnan, but the resistance characteristics of stripe rust are still unclear. In this study, 260 Yunnan landrace wheat germplasm resources preserved in the Crop Genebank were determined by carrying out the identification of stripe rust resistance at adult plant stage in the field and the genotype analysis of three known resistance genes Yr5, Yr10 and Yr15, to clear the resistance level of stripe rust and the distribution of three known resistance genes Yr5, Yr10 and Yr15, which provided a theoretical reference for the screening of excellent resistance resources and the exploration and utilization of resistance genes. The results showed that 38 of 260 Yunnan landrace wheat germplasm resources showed immune and near immune resistance to stripe rust at adult stage in Songming experimental site in 2022, accounting for 14.62 %. There were 93 high resistance materials, accounting for 35.77 %, 46 medium resistance materials, accounting for 17.69 %, and 83 medium and high susceptible materials, accounting for 31.92 %. At the same time, using capillary electrophoresis detection technology, there were 11 materials carrying Yr10 resistance gene in 260 Yunnan landrace wheat germplasm resources, accounting for 4.23 %, and 0 materials carrying Yr5 and Yr15 resistance genes. In summary, 260 Yunnan landrace wheat resources are rich in stripe rust resistance materials. The proportion of immune, near immune and high resistance materials are 50.39 %, and resistance genes Yr5 and Yr15 are undetected. It is speculated that the above materials are likely to carry unknown new resistance genes.

Key words: Yunnan landrace wheat resources, rust disease, resistance identification

CHEN Dan, DU Juan, ZHOU GuoYan, WU XiaoYang, BAI XiaoDong, WU ShaoYun, CAI Qing. Identification of Stripe Rust Resistance and Molecular Detection of Resistance Genes in Yunnan Wheat Landrace Germplasm Resources[J].Journal of Agricultural Big Data, 2023, 5(2): 27-35.

Figures/Tables 6

Table 1

Table 2

Table 3

Table 4

Table 5

Distribution of Yr5, Yr10 and Yr15 gene loci in Yunnan landrace wheat germplasm resources"

供试材料种质编号	Yr5	Yr10	Yr15	供试材料种质编号	Yr5	Yr10	Yr15	供试材料种质编号	Yr5	Yr10	Yr15	供试材料种质编号	Yr5	Yr10	Yr15
供试材料种质编号	wmc175	xpsp3000	barc8	供试材料种质编号	wmc175	xpsp3000	barc8	供试材料种质编号	wmc175	xpsp3000	barc8	供试材料种质编号	wmc175	xpsp3000	barc8
2107				2469				2863				3253
2112				2474				2868				3258
2117				2494				2873				3263
2132				2509				2878				3268
2137				2514				2883				3273
2142				2519				2888				3278
2147				2524				2893				3283
2152				2529				2898				3288
2157				2534				2903				3293
2162				2539				2908				3298
2167				2544				2913				3303
2172				2549				2918				3308
2177				2554				2928				3313
2192				2559				2933				3318
2197				2564				2938				3323
2202				2574				2943				3327
2207				2579				2948				3332
2212				2584				2968				3342
2217				2589				2973				3347
2222				2594				2978				3352
2227				2599				2983				3357
2232				2604				2988				3362
2237				2609				2993				3367
2242				2614				2998				3372
2247				2624				3003				3377
2252				2629		+		3008				3387
2257				2634				3018				3392
2262				2639				3023				3397
2267				2644		+		3028				3407
2272				2649		+		3033				3412
2277				2654				3038				3417
2282				2659				3043				3427
2287				2664				3048				3432
2292				2674				3053				3437
2297				2679				3058				3446
2302				2684				3063				3451
2307				2689				3073				3455
2312				2694				3078				3460
2317				2698				3083				3465
2322				2703				3088				3470
2332				2707				3093				3475
2337				2711				3098				3480
2341				2716				3103				3485
2346				2721				3113				3490
2351				2726				3118				3495
2355				2731				3123				3500
2360				2741				3128				3505
2364				2746				3133				3510		+
2369				2756				3138				3515		+
2374				2761		+		3148				3520		+
2379				2766		+		3153				3524
2384				2770				3158				3529
2394				2775				3163				3534
2399				2784				3168				3539
2404				2799				3173				3544
2409				2809				3178		+		3549
2414				2814				3183				3554
2419				2819				3188				3559
2424				2824				3193				3564
2429				2829				3203				3569		+
2434				2834				3208				3579
2439				2838		+		3213				3584
2444				2843				3218				Avocet S	-	-	-
2449				2848				3223				阳性对照	+	+	+
2459				2853				3238
2464				2858				3243

Table 5

Table 6

References 24

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侵染型	病情指数	抗性评价
0	—	免疫 (I)
0；	—	近免疫 (NIM)
1	—	高抗 (HR)
2	—	中抗 (MR)
3~4	≤25	慢锈 (SR)
3	>25	中感 (MS)
4	>25	高感 (HS)

基因	标记名称	引物序列	连锁距离(cM)	退火温度(℃)	5'修饰	文献
Yr5	wmc175	F:GCTCAGTCAAACCGCTACTTCT R:CACTACTCCAATCTATCGCCGT	1.4、9	60	FAM	[11?-13]
Yr10	xpsp3000	F:GCAGACCTGTGTCATTGGTC R:GATATAGTGGCAGCAGGATACG	1.88	57	HEX	[14]
Yr15	barc8	F:GCGGGAATCATGCATAGGAAAACAGAA R:GCGGGGGCGAAACATACACATAAAAACA	9.00	50	TAMAR	[15]

种质编号 No.	侵染型	普遍率 (%)	平均严重度 (%)	病情指数
2107	1-	100.00	3.00	0.03
2112	1	100.00	1.00	0.01
2117	2	100.00	15.00	0.15
2132	4	100.00	75.00	0.75
2137	3+	95.24	21.00	0.20
2142	2+	31.82	3.00	0.01
2147	0；	0.00	0.00	0
2152	3+	95.65	39.00	0.38
2157	1	66.67	4.00	0.03
2162	3	81.82	31.00	0.25
2167	2+	100.00	13.00	0.13
2172	0	0.00	0.00	0
2177	3+	100.00	40.00	0.40
2192	1-	100.00	2.00	0.02
2197	3+	100.00	65.00	0.65
2202	3+	100.00	0.38	0.38
2207	1-	5.00	0.00	0
2212	0；	0.00	0.00	0
2217	3	100.00	44.00	0.44
2222	1-	75.00	1.00	0.01
2227	1-	75.00	1.00	0.01
2232	4	100.00	91.00	0.91
Avocet S6/Yr5*	0	0.00	0.00	0
Avocet S6/Yr10*	2	8.00	100.00	0.08
Avocet S6/Yr15*	0	0.00	0.00	0
Avocet S	4	100.00	100.00	1

种质资源	抗性类型
种质资源	免疫/近免疫	高抗	中抗	感病	总计
数量	38.00	93.00	46.00	83.00	260
占比（%）	14.62	35.77	17.69	31.92	100

种质资源	携带目标条带类型
种质资源	Yr5	Yr10	Yr15	无带	合计
数量	0	11.00	0	249.00	260
占比（%）	0	4.23	0	95.77	100

Identification of Stripe Rust Resistance and Molecular Detection of Resistance Genes in Yunnan Wheat Landrace Germplasm Resources

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