Journal of Agricultural Big Data ›› 2024, Vol. 6 ›› Issue (1): 14-23.doi: 10.19788/j.issn.2096-6369.100007
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LIU Zhao1,2(), GAO Yuan2, WANG Kun2, SUN SiMiao2, DAI YingZi1, LU Xiang1,2, TIAN Wen1,2, WANG DaJiang2,*(), FENG JianRong1,*()
Received:
2023-12-23
Accepted:
2024-01-26
Online:
2024-03-26
Published:
2024-04-08
LIU Zhao, GAO Yuan, WANG Kun, SUN SiMiao, DAI YingZi, LU Xiang, TIAN Wen, WANG DaJiang, FENG JianRong. Deep Sequencing Dataset of miRNAs in Response to Salt Stress in Apples[J].Journal of Agricultural Big Data, 2024, 6(1): 14-23.
Table 1
Evaluation of sequencing data"
样品处理 Sample processing | 样品名称 Sample name | 原始序列 Raw_reads | 高质量序列 Clean_reads | 质量值 Q30(%) | GC含量 GC(%) |
---|---|---|---|---|---|
NaCl处理0d叶片 | S01 | 13,846,490 | 13,803,489 | 99.27 | 44.59 |
S02 | 20,053,068 | 19,987,801 | 99.18 | 44.33 | |
S03 | 13,248,886 | 13,214,960 | 99.21 | 43.76 | |
NaCl处理0d根系 | S04 | 15,468,076 | 15,424,018 | 99.14 | 46.47 |
S05 | 13,351,285 | 13,302,893 | 99.35 | 47.21 | |
S06 | 10,676,243 | 10,649,229 | 99.20 | 45.67 | |
0.2% NaCl处理4d叶片 | S07 | 12,617,241 | 12,582,648 | 99.11 | 43.12 |
S08 | 15,153,199 | 15,132,214 | 99.28 | 45.30 | |
S09 | 16,511,487 | 16,477,016 | 99.22 | 42.71 | |
0.2% NaCl处理4d根系 | S10 | 17,667,023 | 17,611,616 | 99.15 | 45.62 |
S11 | 17,817,063 | 17,765,179 | 99.26 | 45.92 | |
S12 | 18,629,839 | 18,579,325 | 99.28 | 44.25 | |
0.6% NaCl处理4d叶片 | S13 | 19,289,050 | 19,245,145 | 99.22 | 42.42 |
S14 | 15,140,835 | 15,104,430 | 99.25 | 42.73 | |
S15 | 11,550,839 | 11,525,892 | 99.21 | 42.65 | |
0.6% NaCl处理4d根系 | S16 | 16,644,254 | 16,611,292 | 99.09 | 48.27 |
S17 | 20,776,915 | 20,721,649 | 99.06 | 48.36 | |
S18 | 14,846,340 | 14,790,580 | 99.09 | 49.37 | |
0.2% NaCl处理8d叶片 | S19 | 17,402,258 | 17,376,093 | 99.25 | 41.67 |
S20 | 13,155,001 | 13,123,833 | 99.24 | 42.70 | |
S21 | 14,710,684 | 14,688,830 | 99.25 | 42.16 | |
0.2% NaCl处理8d根系 | S22 | 11,453,041 | 11,420,547 | 99.15 | 45.50 |
S23 | 9,335,753 | 9,305,655 | 99.29 | 47.38 | |
S24 | 10,551,578 | 10,515,517 | 99.13 | 47.30 | |
0.6% NaCl处理8d叶片 | S25 | 11,239,238 | 11,208,905 | 99.26 | 42.80 |
S26 | 15,990,661 | 15,940,683 | 99.16 | 42.96 | |
S27 | 9,393,863 | 9,375,936 | 99.20 | 41.92 | |
0.6% NaCl处理8d根系 | S28 | 12,091,968 | 12,050,134 | 99.10 | 45.49 |
S29 | 14,053,417 | 14,011,022 | 99.34 | 44.95 | |
S30 | 12,830,498 | 12,770,615 | 99.15 | 48.28 | |
0.2% NaCl处理12d叶片 | S31 | 17,753,496 | 17,723,847 | 99.06 | 44.14 |
S32 | 17,291,050 | 17,271,228 | 99.26 | 41.02 | |
S33 | 12,277,158 | 12,239,966 | 99.18 | 43.46 | |
0.2% NaCl处理12d根系 | S34 | 10,725,382 | 10,686,784 | 98.99 | 45.53 |
S35 | 12,884,009 | 12,858,176 | 99.09 | 46.14 | |
S36 | 18,365,765 | 18,289,799 | 99.09 | 46.03 | |
0.6% NaCl处理12d叶片 | S37 | 20,567,162 | 20,521,482 | 99.18 | 43.67 |
S38 | 18,505,328 | 18,469,101 | 99.21 | 43.66 | |
S39 | 20,211,075 | 20,171,756 | 99.16 | 43.69 | |
0.6% NaCl处理12d根系 | S40 | 23,937,701 | 23,858,507 | 99.22 | 45.79 |
S41 | 20,858,808 | 20,809,845 | 99.16 | 46.82 | |
S42 | 21,005,855 | 20,936,321 | 99.24 | 45.92 |
Table 3
Statistics of Target Genes Annotation Results"
靶基因注释数据库 Anno Database | 注释到的数量 Annotated Number | 300≤长度<1000 300≤length<1000 | 长度≥1000 Length≥1000 |
---|---|---|---|
COG_Annotation | 2,363 | 184 | 2,173 |
GO_Annotation | 3,525 | 462 | 3,042 |
KEGG_Annotation | 2,229 | 267 | 1,951 |
KOG_Annotation | 3,390 | 386 | 2,982 |
Pfam_Annotation | 4,899 | 551 | 4,332 |
Swissprot_Annotation | 4,653 | 578 | 4,053 |
eggNOG_Annotation | 5,497 | 722 | 4,748 |
nr_Annotation | 5,690 | 813 | 4,847 |
All_Annotated | 5,692 | 814 | 4,848 |
Table 4
Annotated target genes in response to salt stress"
响应盐胁迫靶基因 Target genes in response to salt stress | 数目 Number |
---|---|
钠/氢反转运蛋白(Na+/H+ Antiporter, NHX) | 5 |
丝氨酸/苏氨酸类蛋白激酶(Mitogen-activated protein kinase, MAPK) | 8 |
钙依赖蛋白激酶(Calcium-dependent protein kinase, CDPK) | 2 |
钙调磷酸酶B样蛋白(Calcineurin B-like proteins, CBLs) | 2 |
类钙调素蛋白(Calmodulin-like proteins, CML) | 5 |
L-抗坏血酸过氧化物酶(Ascorbate peroxidase, APX) | 4 |
富含亮氨酸重复序列延伸蛋白(Leucine-rich repeat extensin, LRX) | 2 |
快速碱化因子(Rapid alkalinization factor, RALF) | 1 |
类受体蛋白激酶(FERONIA, FER) | 6 |
WRKY转录因子 | 21 |
MYB转录因子 | 58 |
NAC转录因子 | 26 |
bHLH转录因子 | 23 |
AP2/ERF转录因子 | 1 |
bZIP转录因子 | 8 |
DREB转录因子 | 3 |
zinc finger转录因子 | 38 |
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